ENVR 431: Techniques in Environmental Health Sciences

Fall, 2006        Call Number  94653    Section  001      Wednesdays, 2:00-4:50 PM          Room  1104 MHRC


This course provides students with a practical knowledge of some of the techniques and assays used routinely in environmental health research and practise.


Principal Instructor

L.M. Ball, McGavran-Greenberg 4114E, 6-7306


Teaching Assistant

Kirk L. Huslage

Textbook

None.  Readings and experimental protocols will either be made available on-line or be handed out with each laboratory session.


Grading

Grades will be based on write-up of each experiment.


Schedule

 Date  Lab  References
  Aug 23  Introduction  
  Aug 30   Bacterial Indicators of Fecal Contamination I Reading/Experimental Protocol
Local Story 1
Economic Impact
EPA Methods
  Sep 06  Bacterial Indicators of Fecal Contamination II  Link to MPN Calculator
  Sep 13  Molecular Biology: 
RT-PCR, Agarose Gel Electrophoresis and Sequence Analysis Used to Investigate a n Enteric Disease Outbreak
   Experimental Protocol
References (3): 
Daniels et al., 2000
Koopmans and Duizer, 2004
Schwab et al., 2000
Bioedit program
Nucleic acid sequence files

  Sep 20 		  S9-mediated Metabolism- Phase I   Experimental Protocol 
  Sep 27  Bacterial Mutagenicity: Ames Assay I   Experimental Protocol
  Oct 04  Bacterial Mutagenicity: Ames Assay II  
  Oct 11   Mammalian Cell Toxicity Assay I   Experimental Protocol
  Oct 18  Mammalian Cell Toxicity Assay II  
  Oct 25   Modeling Demonstration  
  Nov 01  DNA Isolation and Quantitation    Experimental Protocol
  Nov 08  Histology and Pathology  Experimental Protocol
  Nov 15 An Experiment  Experiemtal protocol
  Nov 22  Thanksgiving Break  
  Nov 29  Algal Toxicity Assay I  Experimental Protocol
  Dec 06  Algal Toxicity Assay II