ENVR 431: Techniques in Environmental Health Sciences
Fall, 2007 Call Number 94618 Section 001 Wednesdays, 2:00-4:50 PM Room 1204 MHRC
This
course provides students with a practical knowledge of some of the
techniques and assays used routinely in environmental health research
and practise.
Principal Instructor
L.M. Ball, McGavran-Greenberg
4114E, 6-7306, lmball@unc.edu
Teaching
Assistant
Joseph E. Hatch, hatchjo@email.unc.edu
Textbook
None.
Readings and experimental protocols will either be made available
on-line or be handed out with each laboratory session.
Grading
Grades
will be based on write-up of each experiment.
Schedule
| Date | Lab | References |
| Aug 22 | Introduction | |
| Aug 29 | Bacterial Indicators of Fecal Contamination I | Reading/Experimental
Protocol Local Story 1 Economic Impact EPA Methods |
| Sep 05 | Bacterial Indicators of Fecal Contamination II | Link to MPN
Calculator FDA Excel Spreadsheet |
| Sep 12 | Molecular Biology: RT-PCR, Agarose Gel Electrophoresis and Sequence Analysis Used to Investigate a n Enteric Disease Outbreak For an animation of PCR: www.dnalc.org/ddnalc/resources/pcr.html |
Experimental
Protocol Outbreak Sequences References (3): Daniels et al., 2000 Koopmans and Duizer, 2004 Schwab et al., 2000 Bioedit program Nucleic acid sequence files |
Sep 19 |
S9-mediated Metabolism- Phase I | Experimental Protocol |
| Sep 26 | Bacterial Mutagenicity: Ames Assay I | Experimental Protocol |
| Oct 03 | Bacterial Mutagenicity: Ames Assay II | http://www.bruceames.org/n/about.php http://mcb.berkeley.edu/faculty/BMB/amesb.html |
| Oct 10 | Mammalian Cell Toxicity Assay I | Experimental Protocol |
| Oct 17 | Mammalian Cell Toxicity Assay II | |
| Oct 24 | Modeling Demonstration | Link to ERDEM
program Description of ERDEM |
| Oct 31 | DNA Isolation and Quantitation | Experimental Protocol |
| Nov 07 | Histology and Pathology | Experimental
Protocol H & E Primer |
| Nov 14 | An Experiment | Experiemtal
protocol |
| Nov 22 | Thanksgiving Break | |
| Nov 28 | Algal Toxicity Assay I | Experimental
Protocol |
| Dec 05 | Algal Toxicity Assay II |