Isotope Geochemistry Lab

Beakers:

M: Sarah M
T: Katie M
W: Daven
R:Katie M/Kelsey
F: Arden/Katie H

Zircon lab beakers: Miquela

Filaments:
Miquela/Kelsey
Sean
Katie M-backup

Swiffering:
M/T: Sean
R/F: Sarah M

317/Ordering/Waste disposal: Courtney
312: Josh
Rock crushing lab: Sean

1. take Petri dish from picking lab to Z lab
2. select one hex cap sav. HCS(av) per sample
   1. use HCSav etched with # 1 – 8 from hot plate
3. discard HF/HNO₃ from within HCSav
4. rinse HCSav and HCSav lid with (H₂O)_mQ
5. repeat step 4
6. under microscope inspect WSav to insure it is clean
7. in ethanol, transfer Zs into HCSav using transfer pipette
8. pipette off ethanol
9. add 10 drops (H₂O)_mQ to HCSav
10. pipette off (H₂O)_mQ
11. repeat steps 9 and 10
12. add 20 drops (HF)_z and 2 drops (HNO₃)_z
13. loosely put lid on HCSav
14. add 3 – 6 ml (HF)_g to large Teflon insert
    1. add 1 – 2 ml (HF)_g to small Teflon insert
15. add 1 drop (HNO₃)_z for every ml of HF
16. place HCS(av) into insert
    1. use blue forceps
    2. grasp HCSav by lid
    3. lower into insert
    4. stagger HCSavs so that each lid rests on the other
17. place insert in metal jacket and put in 220°C oven for ~16 hours
    1. do NOT leave for longer than intended

• Disassemble turret:  Screws, springs, ballbearings, retaining ring and screw, and turret handle are not cleaned (store in small beaker).
• Turret blocks go into 2000 mL Pyrex beaker and turret goes into 4000 mL Pyrex beaker.
• Add reagent grade 3.5M HNO₃ to both beakers.
• Boil for an hour on hotplate in the clean lab hood.
• Cool and discard nitric acid into used nitric acid container.
• Rinse 5 times with MQH₂O and discard into used nitric acid container.
• Add ACS-grade hydrogen peroxide (H₂O₂) to both beakers.
• Boil for an hour on hotplate in the clean lab hood.
• Cool and discard hydrogen peroxide into used hydrogen peroxide container.
• Rinse 5 times with MQH₂O and discard into used hydrogen peroxide container.
• Rinse 1 time with methanol and discard methanol into used methanol container.
• Dry in the 70°C oven in the mass spec lab.
• When dry, return to loading bench.

1. 20% HNO3 bath overnight in nalgene bottle
2. Use parafilm to pour HNO3 into clean nalgene
3. Rinse with milli-Q H2O 3 times
4. 20% HCl bath overnight in nalgene bottle
5. Use parafilm to pour HCl into clean nalgene
6. Tap out water and let air dry on kimwipe under one of the work stations

• remove cover slits from filament bricks
• remove filament bricks from inactive turret
• wipe gunk off with aluminum oxide powder, water and q-tip
• wipe gunk off with a Kimwipe (q-tip) soaked in ethanol
• rinse with H₂O_(mQ) 3 times
• add 10% HNO_{3(cleaning grade)} to glass beaker
• place on hotplate and bring to a boil
• discard HNO₃ into glass bottle labeled “discard” or “dirty”
• rinse with H₂O_(mQ) 3 times
• discard H₂O
• quickly move to drying oven overnight
• replace filament bricks onto turret

1. Use the broom by the nitrogen tank to first sweep rightmost and middle labs in the clean mass spec lab. Sweep dirt onto the door mats.  Sweep the lab with the mass spec next and then the lab’s anteroom.  Because there isn’t a door mat, sweep dirt into the hall.
   1. When sweeping the anteroom, either pile up the shoes not in the shoe rack or put them back in said rack.
2. Swifter the labs in the same order as sweeping them.
3. Wet swiffer the labs in the same order that they were dry swiffered and swept.
   1. After wet swiffering a lab, take off the top layer of the door mat in that lab. Place in that lab’s trash bag, remove trash bag and replace with a new one.
   2. Do the same for the middle lab and the lab containing the mass spec.  The anteroom doesn’t have a trash can.
   3. Place the trashbags next to the trash can in the hall by the Men’s bathroom.  If the cleaning of the lab is done on a Friday, take the trash to the dumpster behind Mitchell; we aren’t allowed to have trash sitting out over the weekend.