Welcome to the High Throughput Sequencing Facility of University of North Carolina at Chapel Hill!

We are excited about introducing our facility to UNC Researchers.

HTSF (currently located in Mary Ellen Jones room 920, 921) was established in 2008 with funds from the UCRF, and is supported by LCCC. Its goal is to apply the tools of high throughput sequencing technology to enrich cancer research of UNC scientists.
High throughput sequencing is an extremely fast developing technology, used in many APPLICATIONS (like genome sequencing, genome re-sequencing, chip-seq, cDNA sequencing, digital expression, and microRNA). Our goal is to help you to benefit from this technology in your work.

We have at our disposal 2 different PLATFORMS: Genome Analyzer IIx (GA2) from Illumina (Solexa) and FLX Genome Sequencer from Roche Diagnostics (454).  GA2 is dedicated to deep parallel sequencing of very short sequences (very useful in chip-seq, digital expression etc). FLX Genome system delivers less, but longer sequences (valuable for genomic sequencing or re-sequencing). Having the two types of instruments allows us to produce the data that is the most suitable for you.

We are here to support university research to the best of the abilities of current technology. We are interested in supporting you in your research, and in the developing new technologies, protocols.

On our website you can find information about platforms we are using, applications, protocols, how to use our services (check our User Guide), and what to do with received data (Results and Analysis).

Please, remember, that we are here to help you in developing new fascinating projects – do not hesitate to contact us with any questions or suggestions (including how to improve our website). 

We are looking forward to work with you!

HTSF team