publications

 

ORIGINAL CONTRIBUTIONS


Boulaftali Y, Hess PR, Getz TM, Cholka A, Stolla M, Mackman N, Owens III AP, Ware J, Kahn

ML, Bergmeier W. Platelet ITAM signaling is critical for vascular integrity in inflammation. J

Clin Invest.  2013 Feb 1;123(2):908-16.        


Xiang B, Zhang G, Stefanini L, Bergmeier W, Gartner TK, Whiteheart SW, Li Z. The Src Family

Kinases and Protein Kinase C Synergize to Mediate Gq-dependent Platelet Activation. J Biol

Chem. 2012 Nov 30;287(49):41277-87.


Edelstein LC, Luna EJ, Gibson IB, Bray M, Jin Y, Kondkar A, Nagalla S, Hadjout-Rabi N, Smith TC, Covarrubias D, Jones SN, Ahmad F, Stolla M, Kong X, Fang Z, Bergmeier W, Shaw C, Leal SM, Bray PF. Human Genome-Wide Association and Mouse Knockout Approaches Identify Platelet Supervillin as an Inhibitor of Thrombus Formation under Shear Stress. Circulation, 2012 Jun 5;125(22):2762-71.


Jansen AJ, Josefsson EC, Rumjantseva V, Liu QP, Falet H, Bergmeier W, Cifuni SM, Sackstein R, von Andrian UH, Wagner DD, Hartwig JH, Hoffmeister KM. Desialylation accelerates platelet clearance following refrigeration and initiates GPIbα metalloproteinasemediated cleavage in mice. Blood 2012 Feb 2;119(5) 1263-73.


Stefanini L, Boulaftali Y, Ouellette TD, Holinstat M, Désiré L, Leblond B, Andre P, Conley PB, Bergmeier W. Rap1-Rac1 Circuits Potentiate Platelet Activation. Arterioscler Thromb Vasc Biol. 2012 Feb;32(2):434-41.        


Ahmad F, Boulaftali Y, Greene TK, Ouellette TD, Poncz M, Feske S, Bergmeier W. Relative Contribution of STIM1 and CalDAG-GEFI to Calcium-Dependent Platelet Activation and Thrombosis. J Thromb Haemost. 2011 Oct;9(10):2077-86             


Stadtmann A, Brinkhaus L, Mueller H, Rossaint J, Bolomini-Vittori M, Bergmeier W, Van Aken H, Wagner DD, Laudanna C, Ley K, Zarbock A. Rap1a activation by CalDAG-GEFI and p38 MAPK is involved in E-selectin-dependent slow leukocyte rolling. Eur J Immunol. 2011 Jul;41(7):2074-85.


Hong W, Kondkar AA, Nagalla S, Bergmeier W, Jin Y, Herman JH, Bray PF. Transfection of human platelets with short interfering RNA. Clin Transl Sci. 2011 Jun;4(3):180-2.


Stolla M, Stefanini L, André P, Ouellette TD, Reilly MP, McKenzie SE, Bergmeier W. CalDAG-GEFI deficiency protects mice in a novel model of Fc{gamma}RIIA-mediated thrombosis and thrombocytopenia. Blood. 2011 Jul 28;118(4):1113-20.            


Stolla M, Stefanini L, Roden RC, Hirsch J, Greene T, Ouellette TD, Maloney SF, Diamond SL, Poncz M, Bergmeier W. The kinetics of αIIbβ3 activation determines the size and stability of thrombi in mice: Implications for antiplatelet therapy. Blood 2011, Jan 20; 117 (3):1005-13 


Reilly MP, Sinha U, André P, Taylor SM, Pak Y, DeGuzman FR, Nanda N, Pandey A, Stolla M, Bergmeier W, McKenzie SE. PRT-060318, a novel syk inhibitor, prevents heparin-induced thrombocytopenia and thrombosis in a transgenic mouse model. Blood. 2011 Feb 17;117(7):2241-6.             


Schols S, Cosemans JMEM, Stefanini L, Feijge MAH, Hamulyák K, Deckmyn H, Bergmeier W, Heemskerk JWM. Novel role of glycoprotein Ib-V-IX and von Willebrand factor in platelet activation-dependent fibrin formation at low shear flow. Blood 2011, Jan 13;117(2):651-60.


Canault M, Duerschmied D, Brill A, Stefanini L, Schatzberg D, Cifuni SM, Bergmeier W, Wagner DD. P38 mitogen-activated protein kinase activation during platelet storage: consequences for platelet recovery and hemostatic function in vivo. Blood 2010, Mar 4;115 (9):1835-42


Stefanini L, Roden RC, Bergmeier W. CalDAG-GEFI is at the Nexus of Calcium-dependent Platelet Activation. Blood 2009, Sep 17;114(12):2506-14.


Brill A, Chauhan AK, Canault M, Walsh MT, Bergmeier W, Wagner DD. Oxidative stress activates ADAM17/TACE and induces its target receptor shedding in platelets in a p38- dependent fashion. Cardiovasc Res. 2009 Oct 1;84(1):137-44.


Bergmeier W, Oh-Hora M, McCarl CA, Roden RC, Bray PF, Feske S. R93W mutation in Orai1 causes impaired calcium influx in platelets. Blood 2009 Jan 15;113(3):675-8.           


Cifuni SM, Wagner DD, Bergmeier W. CalDAG-GEFI And Protein Kinase C (PKC) Represent Alternative Pathways Leading To Activation Of Integrin αIIbβ3 In Platelets. Blood 2008 Sep 1; 112(5):1696-703.              


Dole VS, Matuskova J, Vasile E, Yesilaltay A, Bergmeier W, Bernimoulin M, Wagner DD, Krieger M. Thrombocytopenia and Platelet Abnormalities in High-Density Lipoprotein Receptor-Deficient Mice. Arterioscler Thromb Vasc Biol. 2008 Jun;28(6):1111-6


Dole VS, Bergmeier W, Patten IS, Hirahashi J, Mayadas TN, Wagner DD. PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thromb Haemost. 2007 Oct;98(4):806-12.


Bergmeier W, Goerge T, Wang HW, Crittenden JR, Baldwin ACW, Cifuni SM, Housman DE, Graybiel AM, Wagner DD. Mice lacking the signaling molecule, CalDAG-GEFI, represent a model for leukocyte adhesion deficiency type III. J Clin Invest. 2007 Jun;117 (6):1699-707. 


Zhu L, Bergmeier W, Wu J, Jiang H, Stalker TJ, Cieslak M, Fan R, Boumsell L, Kumanogoh A, Kikutan H, Tamagnone L, Wagner DD, Milla ME, Brass LF. Regulated surface expression and shedding support a dual role for semaphorin 4D in platelet responses to vascular injury. Proc Natl Acad Sci U S A 104(5):1621-1626, 2007.


Babic AM, Josefsson EC, Bergmeier W, Wagner DD, Kaufman RM, Silberstein LE, Stossel TP, Hartwig JH, Hoffmeister KM. In vitro function and phagocytosis of galactosylated platelet concentrates after long-term refrigeration. Transfusion 47(3): 442-451, 2007.


Chauhan AK, Kisucka J, Lamb CB, Bergmeier W, Wagner DD. von Willebrand Factor and Factor VIII are independently required to form stable occlusive thrombi in injured veins. Blood. 2007 Mar 15;109(6):2424-9


Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD. The role of platelet adhesion receptor GPIb{alpha} far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A 103(45):16900-16905, 2006.


Chauhan AK, Motto DG, Lamb CB, Bergmeier W, Dockal M, Plaimauer B, Scheiflinger F, Ginsburg D, Wagner DD. Systemic antithrombotic effects of ADAMTS13. J Exp Med 203 (3):767-776, 2006.


Aktas B, Pozagajova M, Bergmeier W, Sunnarborg SW, Offermanns S, Lee DC, Wagner DD, Nieswandt B. Aspirin induces platelet receptor shedding via ADAM17 (TACE). J Biol Chem. 280(48):39716-22, 2005.


Dole VS, Bergmeier W, Mitchell HA, Eichenberger SC, Wagner DD. Activated platelets induce Weibel-Palade body secretion and leukocyte rolling in vivo: Role of P-selectin. Blood 106: 2334-2339, 2005.


Cai S, Dole VS, Bergmeier W, Scafidi J, Feng H, Wagner DD, Davis AE 3rd. A direct role for c1 inhibitor in regulation of leukocyte adhesion. J Immunol 174: 6462-6466, 2005.


Morrell CN, Matsushita K, Chiles K, Scharpf RB, Yamakuchi M, Mason RJ, Bergmeier W, Mankowski JL, Baldwin WM 3rd, Faraday N, Lowenstein CJ. Regulation of platelet granule exocytosis by S-nitrosylation. Proc Natl Acad Sci U S A. 2005 Mar 8;102(10): 3782-7.


Schulze H, Korpal M, Bergmeier W, Italiano Jr, JE, Wahl SM, Shivdasani RA. Interactions between the megakaryocyte/platelet-specific ß1 tubulin and the secretory leukocyte protease inhibitor SLPI suggest a role for regulated proteolysis in platelet functions. Blood, 104(13):3949-57, 2004.


Crittenden JR*, Bergmeier W*, Zhang Y, Piffath CL, Liang Y, Wagner DD, Housman DE, Graybiel AM. Calcium and diacylglycerol signaling through CalDAG-GEFI controls platelet aggregation. Nat Med, 10(9):982-6, 2004.


Bergmeier W, Piffath CL, Cheng G, Dole VS, Zhang Y, von Andrian UH, Wagner DD. Tumor Necrosis Factor-α-Converting Enzyme (ADAM17) Mediates GPIbα Shedding from Platelets In Vitro and In Vivo. Circ Res, 95(7):677-83, 2004.                       


Bergmeier W*, Rabie T*, Strehl A, Piffath CL, Prostredna M, Wagner DD, Nieswandt B. GPVI down-regulation in murine platelets through metalloproteinase-dependent shedding. Thromb Haemost, 91(5):951-958, 2004.


Cambien B, Bergmeier W, Saffaripour S, Wagner DD. Anti-thrombotic activity of TNF-α. J Clin Invest 112: 1589-1596, 2003.


Bergmeier W, Burger PC, Piffath CL, Hoffmeister KM, Hartwig JH, Nieswandt B, Wagner DD. Metalloproteinase inhibitors improve the recovery and hemostatic function of in vitroaged or -injured mouse platelets. Blood 102: 4229-4235, 2003.


Poujol C, Bergmeier W, Nurden P, Nieswandt B, Nurden A. Effect of infusing rat monoclonal antibodies to the murine GPIb-IX-V complex on platelet and megakaryocyte morphology in mice. Platelets. 14:35-45, 2003.


Nieswandt B, Bergmeier W, Schulte V, Takai T, Baumann U, Schmidt RE, Zirngibl H, Bloch W, Gessner JE. Targeting of platelet integrin alphaIIbbeta3 determines systemic reaction and bleeding in murine thrombocytopenia regulated by activating and inhibitory FcgammaR. Int Immunol. 15:341-349, 2003.


Kuijpers MJ, Schulte V, Bergmeier W, Lindhout T, Brakebusch C, Offermanns S, Fassler R, Heemskerk JW, Nieswandt B. Complementary roles of glycoprotein VI and alpha2beta1 integrin in collagen-induced thrombus formation in flowing whole blood ex vivo. Faseb J. 17:685-687, 2003.


Italiano JE, Jr., Bergmeier W, Tiwari S, Falet H, Hartwig JH, Hoffmeister KM, Andre P, Wagner DD, Shivdasani RA. Mechanisms and implications of platelet discoid shape. Blood. 101:4789-4796, 2003.


Hoffmeister KM, Felbinger TW, Falet H, Denis CV, Bergmeier W, Mayadas TN, von Andrian UH, Wagner DD, Stossel TP, Hartwig JH. The clearance mechanism of chilled blood platelets. Cell. 112:87-97, 2003.


Massberg S, Brand K, Gruner S, Page S, Muller E, Muller I, Bergmeier W, Richter T, Lorenz M, Konrad I, Nieswandt B, Gawaz M. A critical role of platelet adhesion in the initiation of atherosclerotic lesion formation. J Exp Med. 196:887-896, 2002.


Bergmeier W, Schulte V, Brockhoff G, Bier U, Zirngibl H, Nieswandt B. Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody. Cytometry. 48:80-86, 2002.


Schulte V, Snell D, Bergmeier W, Zirngibl H, Watson SP, Nieswandt B. Evidence for two distinct epitopes within collagen for activation of murine platelets. J Biol Chem. 276:364-368, 2001.


Nieswandt B, Brakebusch C*, Bergmeier W*, Schulte V, Bouvard D, Mokhtari-Nejad R, Lindhout T, Heemskerk JW, Zirngibl H, Fassler R. Glycoprotein VI but not alpha2beta1 integrin is essential for platelet interaction with collagen. Embo J. 20:2120-2130, 2001.


Nieswandt B, Bergmeier W, Eckly A, Schulte V, Ohlmann P, Cazenave JP, Zirngibl H, Offermanns S, Gachet C. Evidence for cross-talk between glycoprotein VI and Gi-coupled receptors during collagen-induced platelet aggregation. Blood. 97:3829-3835, 2001.


Nieswandt B, Schulte V, Bergmeier W, Mokhtari-Nejad R, Rackebrandt K, Cazenave JP, Ohlmann P, Gachet C, Zirngibl H. Long-term antithrombotic protection by in vivo depletion of platelet glycoprotein VI in mice. J Exp Med. 193:459-469, 2001.


Kauffenstein G, Bergmeier W, Eckly A, Ohlmann P, Leon C, Cazenave JP, Nieswandt B, Gachet C. The P2Y(12) receptor induces platelet aggregation through weak activation of the alpha(IIb)beta(3) integrin - a phosphoinositide 3- kinase-dependent mechanism. FEBS Lett. 505:281-290, 2001.


Bergmeier W, Bouvard D, Eble JA, Mokhtari-Nejad R, Schulte V, Zirngibl H, Brakebusch C, Fassler R, Nieswandt B. Rhodocytin (aggretin) activates platelets lacking alpha(2)beta (1) integrin, glycoprotein VI, and the ligand-binding domain of glycoprotein Ibalpha. J Biol Chem. 276:25121-25126, 2001.


Nieswandt B, Bergmeier W, Schulte V, Rackebrandt K, Gessner JE, Zirngibl H. Expression and function of the mouse collagen receptor glycoprotein VI is strictly dependent on its association with the FcRgamma chain. J Biol Chem. 275:23998-24002, 2000.


Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, Zirngibl H. Identification of critical antigen-specific mechanisms in the development of immune thrombocytopenic purpura in mice. Blood. 96:2520-2527, 2000.


Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. Structural and functional characterization of the mouse von Willebrand factor receptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-893, 2000.


Viitanen P, Lorimer G, Bergmeier W, Weiss C, Kessel M, Goloubinoff P. Purification of mammalian mitochondrial chaperonin 60 through in vitro reconstitution of active oligomers. Methods Enzymol.98:2013-2217, 1999.



REVIEWS


Bergmeier W, Stefanini L. Platelet ITAM signaling. Current Opinion Hematology, in press


Bergmeier W, Weidinger C, Zee I, Feske S. Emerging roles of store-operated Ca ( 2+) entry through STIM and ORAI proteins in immunity, hemostasis and cancer. Channels (Austin). 2013 Mar 19;7(4). [Epub ahead of print]


Stefanini L, Bergmeier W. CalDAG-GEFI and platelet activation. Platelets 2010; 21(4): 239-43


Bergmeier W, Stefanini L. Novel molecules in calcium signaling in platelets. J Thromb Haemost. 2009 Jul;7 Suppl 1:187-90.


Bergmeier W, Chauhan AK, Wagner DD. Glycoprotein Ibalpha and von Willebrand factor in primary platelet adhesion and thrombus formation: lessons from mutant mice. Thromb Haemost. 2008 Feb;99(2):264-70. Review.



BOOK CHAPTERS


Bergmeier W and Hynes RO. Extracellular matrix and thrombosis and hemostasis. Cold Spring Harb Perspect Biol. 2012 Feb 1;4(2).


Bergmeier W and Wagner DD. Inflammation. In: Platelets, 2nd Edition, Michelson, A. (Ed.). San Diego: Elsevier/Academic Press, 2006.


Nieswandt B, Schulte V, and Bergmeier W. Flow cytometric analysis of mouse platelet activation. Methods Mol Biol, 272:255-68, 2004.