Clyde A. HUTCHISON III

EDUCATION:

• Yale University , New Haven, CT; B.S. in Physics, 1960
• California Institute of Technology , Pasadena CA; Ph.D.,1968 in the laboratory of Robert L. Sinsheimer , thesis: "Bacteriophage PhiX174; Viral Genes and Functions"

PROFESSIONAL EXPERIENCE:

• 1968-1973: Assistant Professor, Department of Bacteriology, University of North Carolina at Chapel Hill
• 1968-present: Member, Curriculum in Genetics , UNC-CH
• 1973-1978: Associate Professor, Department of Microbiology and Immunology , UNC-CH
• 1975-1976: Visiting Scientist in the lab of F. Sanger , at theMRC Laboratory of Molecular Biology , Cambridge, England
• 1978-1983: Professor, Department of Microbiology and Immunology, UNC-CH
• 1983-2004: Kenan Professor of Microbiology, University of North Carolina at Chapel Hill
• 1987-1988: Visiting Scientist in the lab of B. G. Barrell , MRC Laboratory of Molecular Biology, Cambridge, England
• 1989-2000: U.S. Editor, DNA Sequence, The Journal of DNA Sequencing and mapping
• 1996-present: Visiting Investigator, The Institute for Genomic Research (TIGR ), Rockville, MD
• 2004-present: Professor Emeritus of Microbiology and Immunology, UNC-CH
• 2004-present: Distinguished Investigator, the J. Craig Venter Institute, Rockville, MD
  

HONORS:

• 1973-1978: Research Career Development Award, NIH (NIAID )
• 1987-1997: NIH MERIT Award, National Institute of Allergy and Infectious Diseases
• 1995-present: member, National Academy of Sciences
• 1995-present: fellow, American Academy of Microbiology
• 1995: North Carolina Award in Science
• 1998-present: fellow, American Academy of Arts and Sciences

SELECTED PUBLICATIONS:

1. Smith, H.O., Hutchison, C.A.III, Pfannkoch, C., and Venter, J.C. (2003). Generating a synthetic genome by whole genome assembly:  phiX174 bacteriophage from synthetic oligonucleotides. Proc. Natl. Acad. Sci. USA 100, 15440-15445.
2. Hutchison, C.A. III, and Montague, M.G.(2002). Mycoplasmas and the minimal genome concept. In Molecular biology and Pathogenicity of Mycoplasmas, pp. 221-253. Razin, S., and Herrmann, R. eds.,  Kluwer Academic/Plenum Publishers.
3. Mears, M.L., and Hutchison, C.A.III (2001). The evolution of modern lineages of mouse L1 elements . J. Mol. Evol. 52, 51-62. [PubMed]
4. Wrobel, J.A., Conrad, M.J., Bloedon, E., Swanstrom, R., and Hutchison, C.A.III (2000). Analysis of HIV-1 reverse transcriptase: comparing sequences of viral isolates with mutational data. AIDS Res. Human Retroviruses 16, 2049-2054. [PubMed] [Supplementary Material]
5. Montague, M.G., and Hutchison, C.A.III (2000). Gene content phylogeny of herpesviruses. Proc. Natl. Acad. Sci. USA 97, 5334-5339.
6. Hutchison, C.A.III, Peterson, S.N., Gill, S.R., Cline, R.T., White, O., Fraser, C.M., Smith, H.O., and Venter, J.C. (1999). Global transposon mutagenesis and a minimal mycoplasma genome. Science 286, 2165-2169.
7. Tomita, M., Hashimoto, K., Takahashi, K., Shimizu T.S., Matsuzaki, Y., Miyoshi, F., Saito, K., Tanida, S., Yugi, K., Venter, J.C., and Hutchison, C.A.III (1999).  E-CELL: software environment for whole-cell simulation. Bioinformatics 15, 72-84 .
8. Wrobel, J.A., Chao, S.-F., Conrad, M.J., Merker, J.D., Swanstrom, R., Pielak, G.J., and Hutchison, C.A.III (1998). A genetic approach for identifying critical residues in the fingers and palm subdomains of HIV-1 reverse transcriptase. Proc. Natl. Acad. Sci. USA 95, 638-645. See also supplementary data .
9. Tollefsbol, T.O., and Hutchison, C.A.III (1997). Control of methylation spreading in synthetic DNA sequences by the murine DNA methyltransferase. J. Mol. Biol. 269, 494-504. [PubMed]
10. Shao, W., Everitt, L., Manchester, M., Loeb, D.D., Hutchison, C.A.III, and Swanstrom, R. (1997). Sequence requirements of the human immunodeficiency virus type 1 protease flap region determined by saturation mutagenesis and kinetic analysis of flap mutants. Proc. Natl. Acad. Sci. USA 94, 2243-2248. [PubMed]
11. Pai, K.S., Bussiere, D.E., Wang, F., Hutchison, C.A.III, White, S.W., and Bastia, D. (1996). The structure and function of the replication terminator protein of Bacillus subtilis: Identification of the &quotwinged helix" DNA-binding domain. EMBO J. 15, 3164-3173. [PubMed]
12. Peterson, S.N., Bailey, C.C., Jensen, J.S., Borre, M.B., King, E.S., Bott, K.F., and Hutchison, C.A. III (1995). Characterization of repetitive DNA in the Mycoplasma genitalium genome: possible role in the generation of antigenic variation.Proc. Natl . Acad. Sci. USA 92, 11829-11833. [PubMed]
13. Fraser, C. M., Gocayne, J. D., White, O., Adams, M. D., Clayton, R. A., Fleischmann, R. D., Bult, C. J., Kerlavage, A. R., Sutton, G., Kelley, J. M., Fritchman, J. L., Weidman, J. F., Small, K. V., Sandusky, M., Fuhrman, J., Utterback, T. R., Saudek, D. M., Phillips, C. A., Merrick, J. N., Tomb, J., Dougherty, B. A., Bott, K. F., Hu, P.-c., Lucier, T. S., Peterson, S. N., Smith, H. O., Hutchison III, C. A. & Venter, J. C. (1995). The minimal gene complement of Mycoplasma genitalium. Science 270, 397-403. [PubMed] Also see TIGR database for M. genitalium.
14. Tollefsbol, T.O., and Hutchison, C.A.III (1995). Mammalian DNA (cytosine-5)-methyltransferase expressed in Escherichia coli, purified and characterized. J. Biol. Chem. 270, 185 43-18550. [PubMed]
15. Chao, S.-F., Chan, V.L., Juranka, P., Kaplan, A.H., Swanstrom, R., and Hutchison, C.A.III (1995). Mutational sensitivity patterns define critical residues in the HIV-1 reverse transcriptase palm domain. Nucl. Acids Res. 23, 803-810. [PubMed] .
16. Davies, C.J., and Hutchison, C.A. III (1995). T Insertion-site specificity of the transposon Tn3 and analysis of "hot" and "cold" spots., Nucl. Acids Res. 23, 507-514.
17. Moody, M.D., Pettitt, S.C., Shao, W., Everitt, L., Loeb, D.D., Hutchison, C.A.III, and Swantrom, R. (1995). A side chain at position 48 of the human immunodeficiency virus type-1 protease flap provides an additional specificity determinant. Virology 2 07, 475-485.
18. Lucier, T.S., Hu, P.-Q., Peterson, S.N., Song, X.-Y., Miller, L., Heitzman, K., Bott, K.F., Hutchison, C.A.III, and Hu, P.-C. (1994). Construction of an ordered genomic library of Mycoplasma genitalium. Gene 150, 27-34.
19. Adey, N.B., Schichman, S.A., Graham, D.K., Peterson, S.N., Edgell, M.H., and Hutchison, C.A. III (1994). Rodent L1 evolution has been driven by a single dominant lineage that has repeatedly acquired new transcriptional regulatory sequences. Mol. Biol. and Evol. 11, 778-789..
20. Manchester, M., Everitt, L., Loeb, D.D., Hutchison, C.A. III, and Swanstrom, R. (1994). Identification of temperature-sensitive mutants of the human immunodeficiency virus type 1 protease through saturation mutagenesis. J. Biol. Chem. 269, 7689-7695.
21. Adey, N.B., Tollefsbol, T.O., Sparks, A.B., Edgell, M.H., and Hutchison, C.A. III (1994). Molecular resurrection of an extinct ancestral promoter for mouse L1. Proc. Natl. Acad. Sci. USA 91, 1569-1573. [PubMed]
22. Peterson, S.N., Hu, P.c., Bott, K.F., and Hutchison, C.A.III (1993). A survey of the Mycoplama genitalium genome using random sequencing. J. Bact. 175, 7918-7930.
23. Vanlerberghe, F., Bonhomme, F., Hutchison, C.A. III, and Edgell, M.H. (1993). A major difference between the divergence patterns within the LINES-1 families in mice and voles. Mol. Biol. and Evol. 10, 719-731.
24. Schichman, S.A., Adey, N.B., Edgell, M.H., and Hutchison, C.A. III (1993). L1 A-monomer tandem arrays have expanded during the course of mouse L1 evolution. Mol. Biol. and Evol. 10, 552-570.
25. Deininger, P.L., Batzer., M., Hutchison, C.A.III, and Edgell, M.H.(1992). Master genes in mammalian repetitive DNA amplification. Trends in Genetics 8, 307-311.
26. Barton, A.B., Davies, C.J., Hutchison, C.A. III, and Kaback, D.B. (1992). Cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the FUN52 gene, whose product has homology to protein kinases. Gene 117, 137-140.
27. Manchester, M., Loeb, D.D., Everitt, L., Moody, M., Hutchison, C.A. III, and Swanstrom, R. (1992). Analysis of temperature-sensitive mutants of the HIV-1 protease. In: Advances in Experimental Medicine and Biology, Dunn, B.M. ed., Plenum Press, New Yo rk, Vol. 306, 493-497.
28. Schichman, S.A., Severynse, D.M., Edgell, M.H., and Hutchison, C.A. III (1992). Strand-specific LINE-1 transcription in mouse F9 cells originates from the youngest phylogenetic subgroup of LINE-1 elements. J. Mol. Biol., 224, 559-574.
29. Severynse, D.M., Hutchison, C.A.III, and Edgell, M.H. (1992). Identification of transcriptional regulatory activity within the 5' A-type monomer sequences of the mouse LINE-1 retroposon. Mammalian Genome, 2, 41-50.
30. Hutchison, C.A. III, Swanstrom, R., and Loeb, D.D. (1991). Complete Mutagenesis of Protein Coding Domains. Methods in Enzymol. 202, 356-390.
31. Bankier, A.T., Beck, S., Bohni, R., Brown, C.M., Cerny, R., Chee, M.S., Hutchison, C.A.III, Kouzarides, T., Martignetti, J.A., Preddie, E., Satchwell, S.C., Tomlinson, P., Weston, K.M., and Barell, B.G. (1991). The DNA sequence of the human cytomegalo virus genome. DNA Sequence 2, 1-12.
32. Peterson, S.N., Schramm, N., Hu, P.-c., Bott, K.F., and Hutchison, C.A. III (1991) A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium. Nucleic Acids Res. 19, 6027-6031.
33. Adey, N.B., Schichman, S.A., Hutchison, C.A. III, and Edgell, M.H. (1991). Composite of A and F-type 5' terminal sequences defines a subfamily of mouse LINE-1 elements. J. Mol. Biol 221, 367-373.
34. Burton, F.H., Loeb, D.D., Edgell, M.H., and Hutchison, C.A. III (1991) L1 Gene conversion or same-site transposition. Mol. Biol. and Evol. 8, 609-619.
35. Hutchison, C.A.III, Hardies, S.C., Loeb, D.D., Shehee, W.R., and Edgell, M.H. (1989). LINES and related retroposons: Long interspersed repeated sequences in the eukaryotic genome. In Mobile DNA, Berg, D.E. and Howe, M.M. eds., Amer. Soc. Microbiol., pp. 593-617.