Vita Weinreb
 

Vita Weinreb, Research Associate, has an MS degree in Electrical Engineering. She previously did DNA sequencing in Oliver Smithies’s laboratory. Since 2000 she has become our biophysical chemist. She purifies and carries out activity and thermal stability measurements on enzymes. Currently, she is programming a Biomek 3000 robot to automate the assays. She enjoys playing keyboard instruments. Her husband, Gabriel is founder and CEO of Simlifes, a consulting firm we use to develop high throughput data collection.

Publications:

1.Chester, A., Weinreb, V., Carter, C. W., Jr. & Navaratnam, N. (2004) Optimization of apolipoprotein B mRNA editing by APOBEC1 apoenzyme and the role of its auxiliary factor, ACF RNA 10, 1399 - 1411.

2.Kapustina, M., Weinreb, V., Li, L., Kuhlman, B. & Carter, C. W., Jr. (2007) A Conformational Transition State Accompanies Amino Acid Activation by B. stearothermphilus Tryptophanyl-tRNA Synthetase Structure 15, 1272-1284.

3.Li, L., Weinreb, V., Francklyn, C. & Carter, C. W., Jr (2011) Histidyl-tRNA Synthetase Urzymes:  Class I and II Aminoacyl-tRNA Synthetase Urzymes have Comparable Catalytic Activities fo Cognate Amino Acid Activation Journal Biologial Chemistry 286, 10387-10395.

4.Pham, Y., Kuhlman, B., Butterfoss, G. L., Hu, H., Weinreb, V. & Carter, C. W., Jr (2010) Tryptophanyl-tRNA synthetase Urzyme:  a model to recapitulate molecular evolution and investigate intramolecular complementation Journal of Biological Chemistry 285.

5.Pham, Y., Li, L., Kim, A., Weinreb, V., Butterfoss, G., Kuhlman, B. & Carter, C. W., Jr (2007) A Minimal TrpRS Catalytic Domain Supports Sense/Antisense Ancestry of Class I and II Aminoacyl-tRNA Synthetases Mol. Cell 25, 851-862.

6.Retailleau, P., Huang, X., Yin, Y., Hu, M., Weinreb, V., Vachette, P., Vonrhein, C., Bricogne, G., Roversi, P., Ilyin, V. & Carter, C. W., Jr. (2003) Interconversion of ATP binding and conformational free energies by Tryptophanyl-tRNA Synthetase:  structures of ATP bound to open and  closed, pre-transition conformations J. Mol. Biol. 325, 39-63.

7.Retailleau, P., Weinreb, V., Hu, M. & Carter, C. W., Jr. (2007) Crystal Structure of Tryptophanyl-tRNA Synthetase Complexed with Adenosine-5' tetraphosphate:  Evidence for Dissociative Character in the Transition State for Amino Acid Activation by Class I Aminoacyl-tRNA Synthetases J. Mol. Biol. 369, 108-128.

8.Weinreb, V. & Carter, C. W., Jr. (2008) Mg2+-free B. stearothermophilus Tryptophanyl-tRNA Synthetase Activates Tryptophan With a Major Fraction of the Overall Rate Enhancement J. Am. Chem. Soc. 130, 1488-1494.

9.Weinreb, V., Kuhlman, B. & Carter, C. W., Jr. (2010) A Master switching motif with multiple effects on differential conformational stability and Mg2+-assisted catalysis suggests a general mechanism for transducing enzymes in American Crystallographic Association Annual Meeting, ed. Angel, R. (American Crystallographic Association, Chicago).

10.Weinreb, V., Li, L. & Carter, C. W., Jr. (2012) A Master Switch Couples Mg2+-Assisted Catalysis to Domain Motion in B. stearothermophilus Tryptophanyl-tRNA Synthetase Structure In Press.

11.Weinreb, V., Li, L., Kaguni, L. S., Campbell, C. L. & Carter, C. W., Jr. (2009) Mg2+-Assisted Catalysis by B. stearothermophilus TrpRS is Promoted by Allosteric Effects Structure 17, 952-964.



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