3D Chromatin Structure
We map DNA loops with high-resolution in human cells progressing through various differentiation processes.Read More
We use LC MS/MS to identify and quantify changes in protein phosphorylation during cellular differentiation process.Read More
We are interested in the complex network of molecular events that drive lineage specification and the maintenance of cellular identity.Read More
We are developing novel software to analyze and visualize data from proteomic and genomic data sets.Read More
Monocytes are multipotent white blood cells that circulate through the vascular system and, upon stimulation, can differentiate into a variety of specialized cell types including macrophages, dendritic cells, and osteoclasts. Mis-regulation of these differentiation processes can lead to disorders including arthritis, atherosclerosis, and leukemia whereas manipulation of these cell fate decisions may offer therapeutic opportunities. The focus of our research is to better understand the molecular mechanisms that drive the transition from monocytes to each of these disease-relevant cell types. We are particularly interested in the phenomena of dynamic DNA looping and kinase signaling and the role that they play in lineage specificity and cell fate decisions. By better understanding the mechanisms that give rise to these cells we can improve our understanding of immune cell development and related disorders. However, the knowledge we gain regarding gene regulation and cell signaling will inform our understanding of a wide variety of biological processes and human diseases. We employ a variety of technologies including genomics, proteomics, genome editing, and bioinformatics to characterize and functionally test molecular events driving monocyte differentiation.