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    Web-review of the RGS & GoLoco proteins
    (c) copyright 2004 David Siderovski & Francis Willard

    References

     

    1. Gilman AG. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987 56:615-49.

    2.  Hamm HE. The many faces of G protein signaling. J Biol Chem. 1998 Jan 9;273(2):669-72.

    3.  Offermanns S. G-proteins as transducers in transmembrane signalling. Prog Biophys Mol Biol. 2003 Oct;83(2):101-30.

    4.  De Vries L, et al. GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11916-20.

    5.  Dohlman HG, et al. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). Mol Cell Biol. 1996 Sep;16(9):5194-209.

    6.  Druey KM, et al. Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature. 1996 Feb 22;379(6567):742-6.

    7.  Koelle MR, et al. EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins. Cell. 1996 Jan 12;84(1):115-25.

    8.  Siderovski DP, et al. A new family of regulators of G-protein-coupled receptors? Curr Biol. 1996 Feb 1;6(2):211-2.

    9.  Yu JH, et al. The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development. Embo J. 1996 Oct 1;15(19):5184-90.

    10.  Berman DM, et al. GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunits. Cell. 1996 Aug 9;86(3):445-52.

    11.  Hunt TW, et al. RGS10 is a selective activator of G alpha i GTPase activity. Nature. 1996 Sep 12;383(6596):175-7.

    12.  Watson N, et al. RGS family members: GTPase-activating proteins for heterotrimeric G-protein alpha-subunits. Nature. 1996 Sep 12;383(6596):172-5.

    13.  Siderovski DP, et al. Whither goest the RGS proteins? Crit Rev Biochem Mol Biol. 1999 34(4):215-51.

    14.  Neubig RR, et al. Regulators of G-protein signalling as new central nervous system drug targets. Nat Rev Drug Discov. 2002 Mar;1(3):187-97.

    15.  Kimple RJ, et al. Established and emerging fluorescence-based assays for G-protein function: heterotrimeric G-protein alpha subunits and regulator of G-protein signaling (RGS) proteins. Comb Chem High Throughput Screen. 2003 Jun;6(4):399-407.

    16.  Arshavsky VY, et al. Lifetime regulation of G protein-effector complex: emerging importance of RGS proteins. Neuron. 1998 Jan;20(1):11-4.

    17.  Brandt DR, et al. GTPase activity of the stimulatory GTP-binding regulatory protein of adenylate cyclase, Gs. Accumulation and turnover of enzyme-nucleotide intermediates. J Biol Chem. 1985 Jan 10;260(1):266-72.

    18.  Higashijima T, et al. Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins. J Biol Chem. 1987 Jan 15;262(2):762-6.

    19.  Chan RK, et al. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol. 1982 Jan;2(1):11-20.

    20.  Chan RK, et al. Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol. 1982 Jan;2(1):21-9.

    21.  von Buchholtz L, et al. RGS21 is a novel regulator of G protein signalling selectively expressed in subpopulations of taste bud cells. Eur J Neurosci. 2004 Mar;19(6):1535-44.

    22.  Wang L, et al. Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring protein. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3220-5.

    23.  Zheng B, et al. Divergence of RGS proteins: evidence for the existence of six mammalian RGS subfamilies. Trends Biochem Sci. 1999 Nov;24(11):411-4.

    24.  Ross EM, et al. GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins. Annu Rev Biochem. 2000 69:795-827.

    25.  Mao H, et al. RGS17/RGSZ2, a novel regulator of Gi/o, Gz, and Gq signaling. J Biol Chem. 2004 Jun 18;279(25):26314-22.

    26.  Jones TLZ. Role of palmitoylation in RGS protein function. Methods Enzymol. 2004 389:33-55.

    27.  Snow BE, et al. A G protein gamma subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gbeta5 subunits. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13307-12.

    28.  Siderovski DP, et al. The GoLoco motif: a Galphai/o binding motif and potential guanine-nucleotide exchange factor. Trends Biochem Sci. 1999 Sep;24(9):340-1.

    29.  Schiff ML, et al. Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel. Nature. 2000 Dec 7;408(6813):723-7.

    30.  Spink KE, et al. Structural basis of the Axin-adenomatous polyposis coli interaction. Embo J. 2000 May 15;19(10):2270-9.

    31.  Kikuchi A. Modulation of Wnt signaling by Axin and Axil. Cytokine Growth Factor Rev. 1999 Sep-Dec;10(3-4):255-65.

    32.  Zheng B, et al. RGS-PX1, a GAP for GalphaS and sorting nexin in vesicular trafficking. Science. 2001 Nov 30;294(5548):1939-42.

    33.  Aiyar A. The use of CLUSTAL W and CLUSTAL X for multiple sequence alignment. Methods Mol Biol. 2000 132:221-41.

    34.  Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996 Aug;12(4):357-8.

    35.  Letunic I, et al. Recent improvements to the SMART domain-based sequence annotation resource. Nucleic Acids Res. 2002 Jan 1;30(1):242-4.

    36.  Kelley LA, et al. Enhanced genome annotation using structural profiles in the program 3D-PSSM. J Mol Biol. 2000 Jun 2;299(2):499-520.

    37.  Snow BE, et al. Fidelity of G protein beta-subunit association by the G protein gamma-subunit-like domains of RGS6, RGS7, and RGS11. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6489-94.

    38.  Siderovski DP, et al. Assays of complex formation between RGS protein G gamma subunit-like domains and G beta subunits. Methods Enzymol. 2002 344:702-23.

    39.  Makino ER, et al. The GTPase activating factor for transducin in rod photoreceptors is the complex between RGS9 and type 5 G protein beta subunit. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1947-52.

    40.  Levay K, et al. Gbeta5 prevents the RGS7-Galphao interaction through binding to a distinct Ggamma-like domain found in RGS7 and other RGS proteins. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2503-7.

    41.  Witherow DS, et al. Complexes of the G protein subunit gbeta 5 with the regulators of G protein signaling RGS7 and RGS9. Characterization in native tissues and in transfected cells. J Biol Chem. 2000 Aug 11;275(32):24872-80.

    42.  van der Linden AM, et al. The G-protein beta-subunit GPB-2 in Caenorhabditis elegans regulates the G(o)alpha-G(q)alpha signaling network through interactions with the regulator of G-protein signaling proteins EGL-10 and EAT-16. Genetics. 2001 May;158(1):221-35.

    43.  Chase DL, et al. Two RGS proteins that inhibit Galpha(o) and Galpha(q) signaling in C. elegans neurons require a Gbeta(5)-like subunit for function. Curr Biol. 2001 Feb 20;11(4):222-31.

    44.  Sondek J, et al. Ggamma-like (GGL) domains: new frontiers in G-protein signaling and beta-propeller scaffolding. Biochem Pharmacol. 2001 Jun 1;61(11):1329-37.

    45.  Jones MB, et al. The Gbeta/gamma dimer as a novel source of selectivity in G-protein signaling: GGL-ing at convention. Mol Interv. 2004 Aug;4:200-14.

    46.  Ponting CP, et al. Pleckstrin's repeat performance: a novel domain in G-protein signaling? Trends Biochem Sci. 1996 Jul;21(7):245-6.

    47.  Hu G, et al. R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9755-60.

    48.  Lishko PV, et al. Specific binding of RGS9-Gbeta 5L to protein anchor in photoreceptor membranes greatly enhances its catalytic activity. J Biol Chem. 2002 Jul 5;277(27):24376-81.

    49.  Hu G, et al. Activation of RGS9-1GTPase acceleration by its membrane anchor, R9AP. J Biol Chem. 2003 Apr 18;278(16):14550-4.

    50.  Martemyanov KA, et al. The DEP domain determines subcellular targeting of the GTPase activating protein RGS9 in vivo. J Neurosci. 2003 Nov 12;23(32):10175-81.

    51.  Nishiguchi KM, et al. Defects in RGS9 or its anchor protein R9AP in patients with slow photoreceptor deactivation. Nature. 2004 Jan 1;427(6969):75-8.

    52.  Oliveira-Dos-Santos AJ, et al. Regulation of T cell activation, anxiety, and male aggression by RGS2. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12272-7.

    53.  Tang MK, et al. Regulator of G-protein signaling-2 mediates vascular smooth muscle relaxation and blood pressure. Nat Med. 2003 Dec;9(12):1506-12.

    54.  Heximer SP, et al. Hypertension and prolonged vasoconstrictor signaling in RGS2-deficient mice. J Clin Invest. 2003 Feb;111(4):445-52.

    55.  Grant SL, et al. Specific regulation of RGS2 messenger RNA by angiotensin II in cultured vascular smooth muscle cells. Mol Pharmacol. 2000 Mar;57(3):460-7.

    56.  Snow BE, et al. GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain. J Biol Chem. 1998 Jul 10;273(28):17749-55.

    57.  Snow BE, et al. Molecular cloning of regulators of G-protein signaling family members and characterization of binding specificity of RGS12 PDZ domain. Methods Enzymol. 2002 344:740-61.

    58.  Forman-Kay JD, et al. Diversity in protein recognition by PTB domains. Curr Opin Struct Biol. 1999 Dec;9(6):690-5.

    59.  Zeng W, et al. The N-terminal domain of RGS4 confers receptor-selective inhibition of G protein signaling. J Biol Chem. 1998 Dec 25;273(52):34687-90.

    60.  Xu X, et al. RGS proteins determine signaling specificity of Gq-coupled receptors. J Biol Chem. 1999 Feb 5;274(6):3549-56.

    61.  Ingi T, et al. Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity. J Neurosci. 1998 Sep 15;18(18):7178-88.

    62. Wang Q, et al. Receptor-selective effects of endogenous RGS3 and RGS5 regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells. J Biol Chem. 2002 Jul 12;277(28):24949-58.

    63.   Bernstein LS, et al. RGS2 binds directly and selectively to the M1 muscarinic acetylcholine receptor third intracellular loop to modulate Gq/11alpha signaling. J Biol Chem. 2004 May 14;279(20):21248-56.

    64.  Chen JG, et al. A seven-transmembrane RGS protein that modulates plant cell proliferation. Science. 2003 Sep 19;301(5640):1728-31.

    65.  Willard FS, et al. Purification and in vitro functional analysis of the Arabidopsis thaliana Regulator of G-Protein Signaling-1. Methods Enzymol. 2004 389:320-37.

    66.  Chen J-G, et al. AtRGS1 function in Arabidopsis thaliana. Methods Enzymol. 2004 389:338-50.

    67.  Hepler JR, et al. RGS4 and GAIP are GTPase-activating proteins for Gq alpha and block activation of phospholipase C beta by gamma-thio-GTP-Gq alpha. Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):428-32.

    68.  Carman CV, et al. Selective regulation of Galpha(q/11) by an RGS domain in the G protein-coupled receptor kinase, GRK2. J Biol Chem. 1999 Nov 26;274(48):34483-92.

    69.  Sallese M, et al. Selective regulation of Gq signaling by G protein-coupled receptor kinase 2: direct interaction of kinase N terminus with activated galphaq. Mol Pharmacol. 2000 Apr;57(4):826-31.

    70.  Anger T, et al. Differential contribution of GTPase activation and effector antagonism to the inhibitory effect of RGS proteins on Gq-mediated signaling in vivo. J Biol Chem. 2004 Feb 6;279(6):3906-15.

    71.  Kaibuchi K, et al. Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells. Annu Rev Biochem. 1999 68:459-86.

    72.  Sah VP, et al. The role of Rho in G protein-coupled receptor signal transduction. Annu Rev Pharmacol Toxicol. 2000 40:459-89.

    73.  Snyder JT, et al. Structural basis for the selective activation of Rho GTPases by Dbl exchange factors. Nat Struct Biol. 2002 Jun;9(6):468-75.

    74.  Kozasa T, et al. p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. Science. 1998 Jun 26;280(5372):2109-11.

    75.  Hart MJ, et al. Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13. Science. 1998 Jun 26;280(5372):2112-4.

    76.  Booden MA, et al. Leukemia-associated Rho guanine nucleotide exchange factor promotes G alpha q-coupled activation of RhoA. Mol Cell Biol. 2002 Jun;22(12):4053-61.

    77.  Vogt S, et al. Receptor-dependent RhoA activation in G12/G13-deficient cells: genetic evidence for an involvement of Gq/G11. J Biol Chem. 2003 Aug 1;278(31):28743-9.

    78. Suzuki N, et al. Galpha 12 activates Rho GTPase through tyrosine-phosphorylated leukemia-associated RhoGEF. Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):733-8.

    79.  Chikumi H, et al. Regulation of G protein-linked guanine nucleotide exchange factors for Rho, PDZ-RhoGEF, and LARG by tyrosine phosphorylation: evidence of a role for focal adhesion kinase. J Biol Chem. 2002 Apr 5;277(14):12463-73.

    80.  Wang Q, et al. Thrombin and Lysophosphatidic Acid Receptors Utilize Distinct rhoGEFs in Prostate Cancer Cells. J Biol Chem. 2004 Jul 9;279(28):28831-4.

    81.  Taya S, et al. Direct interaction of insulin-like growth factor-1 receptor with leukemia-associated RhoGEF. J Cell Biol. 2001 Nov 26;155(5):809-20.

    82.  Becknell B, et al. Characterization of leukemia-associated Rho guanine nucleotide exchange factor (LARG) expression during murine development. Cell Tissue Res. 2003 Dec;314(3):361-6.

    83. Kumanogoh A, et al. Biological functions and signaling of a transmembrane semaphorin, CD100/Sema4D. Cell Mol Life Sci. 2004 Feb;61(3):292-300.

    84.  Aurandt J, et al. The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG. Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12085-90.

    85. Driessens MH, et al. B plexins activate Rho through PDZ-RhoGEF. FEBS Lett. 2002 Oct 9;529(2-3):168-72.

    86.  Hirotani M, et al. Interaction of plexin-B1 with PDZ domain-containing Rho guanine nucleotide exchange factors. Biochem Biophys Res Commun. 2002 Sep 13;297(1):32-7.

    87.  Perrot V, et al. Plexin B regulates Rho through the guanine nucleotide exchange factors leukemia-associated Rho GEF (LARG) and PDZ-RhoGEF. J Biol Chem. 2002 Nov 8;277(45):43115-20.

    88.  Swiercz JM, et al. Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulate RhoA and growth cone morphology. Neuron. 2002 Jul 3;35(1):51-63.

    89.  Hunt RA, et al. Snapin interacts with the N-terminus of regulator of G protein signaling 7. Biochem Biophys Res Commun. 2003 Apr 4;303(2):594-9.

    90.  Takesono A, et al. Receptor-independent activators of heterotrimeric G-protein signaling pathways. J Biol Chem. 1999 Nov 19;274(47):33202-5.

    91.  Willard FS, et al. Return of the GDI: The GoLoco motif in cell division. Annu Rev Biochem. 2004 73:925-51.

    92.  Kimple RJ, et al. Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits. Nature. 2002 Apr 25;416(6883):878-81.

    93.  Peterson YK, et al. Stabilization of the GDP-bound conformation of Gialpha by a peptide derived from the G-protein regulatory motif of AGS3. J Biol Chem. 2000 Oct 27;275(43):33193-6.

    94.  Kimple RJ, et al. Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif. Biochem J. 2004 Mar 15;378(Pt 3):801-8.

    95.  Snow BE, et al. Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun. 1997 Apr 28;233(3):770-7.

    96.  Kimple RJ, et al. RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity. J Biol Chem. 2001 Aug 3;276(31):29275-81.

    97.  Hollinger S, et al. RGS14 is a bifunctional regulator of Galphai/o activity that exists in multiple populations in brain. J Neurochem. 2001 Dec;79(5):941-9.

    98.  Traver S, et al. RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao. Biochem J. 2000 Aug 15;350 Pt 1:19-29.

    99.  Mochizuki N, et al. Identification and cDNA cloning of a novel human mosaic protein, LGN, based on interaction with G alpha i2. Gene. 1996 Nov 28;181(1-2):39-43.

    100.  Schaefer M, et al. A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila. Curr Biol. 2000 Apr 6;10(7):353-62.

    101.  Yu F, et al. Analysis of partner of inscuteable, a novel player of Drosophila asymmetric divisions, reveals two distinct steps in inscuteable apical localization. Cell. 2000 Feb 18;100(4):399-409.

    102.  Colombo K, et al. Translation of polarity cues into asymmetric spindle positioning in Caenorhabditis elegans embryos. Science. 2003 Jun 20;300(5627):1957-61.

    103.  Srinivasan DG, et al. A complex of LIN-5 and GPR proteins regulates G protein signaling and spindle function in C elegans. Genes Dev. 2003 May 15;17(10):1225-39.

    104.  Gotta M, et al. Asymmetrically distributed C. elegans homologs of AGS3/PINS control spindle position in the early embryo. Curr Biol. 2003 Jun 17;13(12):1029-37.

    105.  Oberdick J, et al. A Purkinje cell differentiation marker shows a partial DNA sequence homology to the cellular sis/PDGF2 gene. Neuron. 1988 Jul;1(5):367-76.

    106.  Mochizuki N, et al. Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with G alpha(i). Nature. 1999 Aug 26;400(6747):891-4.

    107.  Natochin M, et al. Inhibition of GDP/GTP exchange on G alpha subunits by proteins containing G-protein regulatory motifs. Biochemistry. 2001 May 1;40(17):5322-8.

    108.  Meng J, et al. Functional interaction between Galpha(z) and Rap1GAP suggests a novel form of cellular cross-talk. J Biol Chem. 1999 Dec 17;274(51):36663-9.

    109.  Meng J, et al. Activation of Gz attenuates Rap1-mediated differentiation of PC12 cells. J Biol Chem. 2002 Nov 8;277(45):43417-24.

    110.  Jordan JD, et al. Modulation of rap activity by direct interaction of Galpha(o) with Rap1 GTPase-activating protein. J Biol Chem. 1999 Jul 30;274(31):21507-10.

    111.   Ma H, et al. Influence of cytosolic AGS3 on receptor--G protein coupling. Biochemistry. 2003 Jul 8;42(26):8085-93.

    112.   Sato M, et al. AGS3 and signal integration by Galpha(s)- and Galpha(i)-coupled receptors: AGS3 blocks the sensitization of adenylyl cyclase following prolonged stimulation of a Galpha(i)-coupled receptor by influencing processing of Galpha(i). J Biol Chem. 2004 Apr 2;279(14):13375-82.

    113.   Bowers MS, et al. AGS3: a G-Protein regulator of addiction-associated behaviors. Ann N Y Acad Sci. 2003 Nov;1003:356-7.

    114.   Kimple RJ, et al. The GoLoco motif: heralding a new tango between G protein signaling and cell division. Mol Interv. 2002 Apr;2(2):88-100.

    115.  Ghosh M, et al. Receptor- and nucleotide exchange-independent mechanisms for promoting G protein subunit dissociation. J Biol Chem. 2003 Sep 12;278(37):34747-50.

    116.  Oxford GS, et al. GoLoco motif peptides as probes of G-alpha subunit specificity in coupling of GPCRs to ion channels. Methods Enzymol. 2004 390:437-50.

    117.  Knoblich JA. Asymmetric cell division during animal development. Nat Rev Mol Cell Biol. 2001 Jan;2(1):11-20.

    118.  Schober M, et al. Bazooka recruits Inscuteable to orient asymmetric cell divisions in Drosophila neuroblasts. Nature. 1999 Dec 2;402(6761):548-51.

    119.  Wodarz A, et al. Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts. Nature. 1999 Dec 2;402(6761):544-7.

    120.  Kraut R, et al. Role of inscuteable in orienting asymmetric cell divisions in Drosophila. Nature. 1996 Sep 5;383(6595):50-5.

    121.  Knoblich JA, et al. Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization. Curr Biol. 1999 Feb 11;9(3):155-8.

    122.   Tio M, et al. A functional analysis of inscuteable and its roles during Drosophila asymmetric cell divisions. J Cell Sci. 1999 May;112 (Pt 10):1541-51.

    123.  Parmentier ML, et al. Rapsynoid/partner of inscuteable controls asymmetric division of larval neuroblasts in Drosophila. J Neurosci. 2000 Jul 15;20(14):RC84.

    124.  Schaefer M, et al. Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system. Cell. 2001 Oct 19;107(2):183-94.

    125.  Fuse N, et al. Heterotrimeric G proteins regulate daughter cell size asymmetry in Drosophila neuroblast divisions. Curr Biol. 2003 May 27;13(11):947-54.

    126.   Macara IG. Parsing the polarity code. Nat Rev Mol Cell Biol. 2004 Mar;5(3):220-31.

    127.  Gotta M, et al. Distinct roles for Galpha and Gbetagamma in regulating spindle position and orientation in Caenorhabditis elegans embryos. Nat Cell Biol. 2001 Mar;3(3):297-300.

    128.  Grill SW, et al. The distribution of active force generators controls mitotic spindle position. Science. 2003 Jul 25;301(5632):518-21.

    129.  Gonczy P, et al. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature. 2000 Nov 16;408(6810):331-6.

    130. Blatch GL, et al. The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. Bioessays. 1999 Nov;21(11):932-9.

    131.  Miller KG, et al. A genetic selection for Caenorhabditis elegans synaptic transmission mutants. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12593-8.

    132.  Miller KG, et al. RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system. Neuron. 2000 Aug;27(2):289-99.

    133.  Miller KG, et al. A role for RIC-8 (Synembryn) and GOA-1 (G(o)alpha) in regulating a subset of centrosome movements during early embryogenesis in Caenorhabditis elegans. Genetics. 2000 Dec;156(4):1649-60.

    134. Tall GG, et al. Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor. J Biol Chem. 2003 Mar 7;278(10):8356-62.

    135.  Ferguson KM, et al. The influence of bound GDP on the kinetics of guanine nucleotide binding to G proteins. J Biol Chem. 1986 Jun 5;261(16):7393-9.

    136. Plusa B, et al. Site of the previous meiotic division defines cleavage orientation in the mouse embryo. Nat Cell Biol. 2002 Oct;4(10):811-5.

    137.  Cayouette M, et al. Asymmetric segregation of Numb in retinal development and the influence of the pigmented epithelium. J Neurosci. 2001 Aug 1;21(15):5643-51.

    138.  Zhong H, et al. A spatial focusing model for G protein signals. Regulator of G protein signaling (RGS) protien-mediated kinetic scaffolding. J Biol Chem. 2003 Feb 28;278(9):7278-84.

    139.  Saitoh O, et al. RGS8 accelerates G-protein-mediated modulation of K+ currents. Nature. 1997 Dec 4;390(6659):525-9.

    140.  Doupnik CA, et al. RGS proteins reconstitute the rapid gating kinetics of gbetagamma-activated inwardly rectifying K+ channels. Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10461-6.

    141. Mukhopadhyay S, et al. Quench-flow kinetic measurement of individual reactions of G-protein-catalyzed GTPase cycle. Methods Enzymol. 2002 344:350-69.

    142.  McEwen DP, et al. Fluorescence approaches to study G protein mechanisms. Methods Enzymol. 2002 344:403-20.

    143.  Carlier MF. Role of nucleotide hydrolysis in the dynamics of actin filaments and microtubules. Int Rev Cytol. 1989 115:139-70.

    144.  Erickson HP, et al. Microtubule dynamic instability and GTP hydrolysis. Annu Rev Biophys Biomol Struct. 1992 21:145-66.

    145.  Wang N, et al. Tubulin binds specifically to the signal-transducing proteins, Gs alpha and Gi alpha 1. J Biol Chem. 1990 Jan 25;265(3):1239-42.

    146.  Rasenick MM, et al. Exchange of guanine nucleotides between tubulin and GTP-binding proteins that regulate adenylate cyclase: cytoskeletal modification of neuronal signal transduction. J Neurochem. 1988 Jul;51(1):300-11.

    147.  Roychowdhury S, et al. G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements. Biochemistry. 1993 May 11;32(18):4955-61.

    148.  Yan K, et al. Tubulin stimulates adenylyl cyclase activity in C6 glioma cells by bypassing the beta-adrenergic receptor: a potential mechanism of G protein activation. J Neurochem. 2001 Jan;76(1):182-90.

    149.   Chen NF, et al. A specific domain of Gialpha required for the transactivation of Gialpha by tubulin is implicated in the organization of cellular microtubules. J Biol Chem. 2003 Apr 25;278(17):15285-90.

    150.  Roychowdhury S, et al. G protein alpha subunits activate tubulin GTPase and modulate microtubule polymerization dynamics. J Biol Chem. 1999 May 7;274(19):13485-90.

    151.  Walker RA, et al. Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. J Cell Biol. 1988 Oct;107(4):1437-48.

    152.  Labbe JC, et al. PAR proteins regulate microtubule dynamics at the cell cortex in C. elegans. Curr Biol. 2003 Apr 29;13(9):707-14.

    153.  Mountain V, et al. Dissecting the role of molecular motors in the mitotic spindle. Anat Rec. 2000 Feb 15;261(1):14-24.

    154.  Hunter AW, et al. How motor proteins influence microtubule polymerization dynamics. J Cell Sci. 2000 Dec;113 Pt 24:4379-89.