Cell lines and reagents:

DLD-1: RPMI-1640, 10%FBS, L-glu, P/S

U20S: DMEM, 10%FBS, L-glu, P/S (hygromycin for insert if applicable)

HCT-116: McCoy’s5A, 10%FBS, Gentamicin

LS-174T: EMEM, 10%FBS, L-glu, Na-pyruvate, P/S

SW-480’s: IMEM, 10%FBS, gentamicin,

Or EMEM, 15%FBS, Na-pyruvate

HCA-7: DMEM, 10% FBS, P/S

CMT-93: DMEM, 10%FBS

HEK293: DMEM, 10%FBS, P/S, (blasticidin for insert if applicable)

PC-3: Complete RPMI, 10%FBS

HCT-15: RPMI-1640 (gibco’s 31800), 2mM L-glu, Na-pyruvate, gentamicin 10%FBS

A549: RPMI-1640, 2% L-glu, gentamicin Or Ham’s F12 or F12K?

HeLa: EMEM, 10%FBS, 2mM L-glu, P/S

SF9: SF9 media plus 5ml P/S, gungizone, pleuronic acid, 10%FBS, 1 packet.

MCF-7: DMEM, 10%FBS, P/S

Na-pyruvate 100X=100mM; Gentamicin 1000X=10mg/ml; P/S 100X=10,000U/ml;

L-glu 100X=200mM