Protocol for 32P Random Priming Labeling:
									
Using the BM Random Primed Labeling Kit:

Reagents: Fresh 32P dCTP, locking Eppendorf tubes, cDNA, hot water bath,
dH2O, ice, dNTP's, reaction mixture #6, Klenow Enzyme vial #7, 37'C incubator.

1. To a locking Eppendorf tube add 8.0ul dH2O, then 2ul cDNA(or as appropriate)
 for a total of 10ul. Vortex mixture.
2. Denature for 10 minutes in a boiling water bath. Vortex briefly.
3. Place on ice for 10 minutes. Wipe off and vortex briefly.
4. Spin down tube briefly.
5. In order, add the following reagents to the sample while they are all stored on ice:
	dNTP's (G,A,T)			           3.0ul
	Reaction mixture #6		    2.0ul
	32P dCTP behind shield	5.0ul
	Klenow Enzyme vial #7	1.0ul 
  (never let enzyme get to RT!)
6. Vortex mixture very well with Rad shield in place.
7. Incubate at 37'C at Rad desk with Rad shield in place for1 hour.