make a fresh stock--we find that a 6x solution lasts longer before precipitating)</I>

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<H3>Older (pre-Long Ranger Gels) Method</H3>
10X EB
<dl>
<dt>164.0 g Tris-OH
<dt>27.5 g Boric Acid
<dt>7.45 g disodium EDTA
<dt>water to 1 l</dl><p>

Acrylamide stock
<dl>
<dt>38%         Acrylamide
<dt>2%   bis-acrylamide</dl><p>

Gels (volume is for standard size sequencing gels)

<dl> <dt>       <b>8% gel:</b>
<dt>    40.4 g  urea
<dt>27.0 ml     water
<dt>16.8 ml     38/2 acrylamide
<dt>8.0 ml      EB<p>
        
<dt><b>6% gel:</b>      
<dt>40.4 g      urea
<dt>31.2 ml     water
<dt>12.6 ml     38/2 acrylamide
<dt>8.0 ml      EB<p>

<dt><b>5% gel:</b>      
<dt>40.4 g      urea
<dt>33.5 ml     water
<dt>10.5 ml     38/2 acrylamide
<dt>8.0 ml      EB</dl><p>

Dissolve by heating slightly, degas if necessary.  Add 0.7 ml 10% ammonium persulfate, and 25 ul TEMED, and pour gel immediately.
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