Molecular Biology Protocols for Fellow Lab Rats Molecular biology protocols for Fellow Lab Rats I have developed most if not all of these protocols while at NIEHS, UNC, or Duke. They all work well for me, but be sure to get additional information if you are not familiar with the Intricacies of them.
Tissue Culture
  1. Cell lines and reagents I have used
  2. FREEZING CELLS
  3. TYPE II CELL PREP FROM RAT LUNGS
  4. MONOCYTE ISOLATION
  5. PERIPHERAL BLOOD CELL ISOLATION
  6. ROSETTE
  7. COLORECTAL CELL LINES
  8. ISOLATION OF PIG CELLS
    Enzymes Stuff
  9. COX-2 Semiprotein Preparation
  10. Oxygraph Setup
    DNA Techniques
  11. EMSA
  12. PCR VENT TAQ
  13. PCR PLATNIUM TAQ
  14. SYBR GREEN PCR
  15. RT SYBR GREEN
  16. Commonly used dual labelled probes.html
  17. DIG RNA
  18. ELECTROPHORESES
  19. LABELING
  20. OLIGO PREPERATION
  21. SEQUENCING SETUP
  22. SEQUENCING GELS
  23. TOPO Cloning
  24. Transformation
    RNA and Reverse Transcription
  25. SUPER SCRIPT II RT
  26. SUPER SCRIPT RT
  27. QIAGEN OMNISCRIPT RT
  28. NORTHERN BLOTTING
  29. RNA ISOLATION THE OLD FASHION WAY
  30. RIBONUCLEASE PROTECTION ASSAY
    Proteins
  31. VEGF ELISA Using Kit
  32. VEGF ELISA Quantikine.pdf file
  33. PAGE
  34. BCA MICROPLATES
  35. Whole Cell Lysates from Monolayer Cultures
  36. Cell membrane preparation
  37. PAGE NOVEX
  38. PROTEIN ISOLATION FROM TISSUE
  39. PROTEIN ISOLATION FROM MONOLAYER
  40. PROTEIN ISOLATION FROM TRIZOL
  41. SDS PAGE
  42. Nuclear Extracts
  43. WESTERN BLOTTING
    In situ
  44. tunnel staining for Apoptosis
  45. H and E staining
    Other Stuff for My Laboratory
  46. SUPPLIES
  47. LIST OF REAGENTS
  48. restriction table.html
  49. HPLC