General Protocols

Competent Cells

General Lab Solutions

RT PCR Protocol

Cloning of Oligos Into Plasmids

90 Second Transformation

Whole-Mount in situ Hybridization

Tbx20 whole-mount in situ hybridization

In Situ Checklist

Whole-Mount Antibody Staining

Dan Weeks Whole-Mount Antibody Staining

Cryosectioning Xenopus Embryos

St. 37 Xenopus heart, stained with anti-tropomyosin and DAPI

Immunostaining of sectioned embryos

Benzidine Staining of Hemoglobin

Benzidine stain revealing blood in a Xenopus embryo

Xenopus laevis in vitro fertilization method

Transgenesis Protocol

TUNEL Staining of Whole Xenopus Embryos

Protein Protocols

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Whole Embryo Protein

Extraction with Freon

SDS-PAGE gels

Western Protocol

Morpholinos

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Tbx20 Morpholino Information

Tbx5 Morpholino Information

CST Morpholinos

Xenopus Tropicalis Protocols

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Tail Sampling for Genomic DNA Purification

Protocal for Anaesthesia, In Vitro Fertilization, and Natural Mating of Xenopus Tropicalis

Natural mating of X. tropicalis

Mouse Protocols

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