Steven C. Hoffmann

201 Highway 54 Bypass
Carrboro, NC 27510
(919) 942-3517
torch@med.unc.edu


EDUCATION
M.S., Pathology May 1995
Department of Pathology
University of North Carolina at Chapel Hill
Chapel Hill, North Carolina

B.S., Biochemistry (Minor: Chemistry), June 1989
Department of Biochemistry and Biophysics
University of Pittsburgh
Pittsburgh, Pennsylvania
PROFESSIONAL EXPERIENCE

Research Specialist
Division of Cardiothoracic Surgery
University of North Carolina at Chapel Hill
Chapel Hill, NC
January 1996-present
Perform clinical research on rat and pig models of lung ischemia/reperfusion injury and lung transplantation. Responsibilities include small and large animal surgery, isolated lung perfusion, HPLC, immunohistochemistry of endothelial adhesion molecules and immunoassays of cell metabolites. Perform surgical assistance in sheep model establishing Fontan physiology for pediatric congenital heart defects

Plan and Direct research investigating lung capillary bed integrity, drug induced interventions, cytokine regulation and adhesion molecule regulation at the cellular and molecular level. Performed RNA/mRNA isolations, hemodynamic monitoring, graphical and statistical analysis, laboratory webpage design and maintenance of all laboratory equipment.

Supervise all aspects of lab management including planning and coordination of experiments, IACUC protocol development and adherence to all GMP and GLP policies. Responsible for oral and written presentations, manuscript and grant writing and preparation. Instruct and supervise multiple visiting Research Fellows, the first-year Cardiothoracic Surgery Fellow, several medical and undergraduate students in research design, application and all laboratory procedures and methodology

Research Assistantship
Environmental Pathology Training Grant
Sponsor: National Institute of Environmental Health Sciences
Department of Pathology
University of North Carolina at Chapel Hill
August 1990-May 1995
Planned thesis experiments in determining altered expression of p53 tumor suppressor gene in rat liver carcinogenesis model and suppressive effects of exogenous transfection of self-designed wild-type p53 expression construct

Completed Masters Thesis and 54 graduate credit hours of instruction in Pathology MS/PhD program with emphasis in genetics and molecular biology with high passing or honors

Performed lab procedures in molecular biology such as northern and Southern blotting/hybridization, radioassays and probes, gene targeting, vector construction, animal surgery, tissue culture techniques, PCR, nucleic acid and protein extraction and SDS-PAGE electrophoresis and analyzed data from laboratory records with knowledge in current statistical and graphic software packages

Supervised and conducted pathology lab review and techniques for fellow graduate students and technicians including oral presentation of relevent or thesis research

Research Assistant II
Eye and Ear Institute of Pittsburgh
Department of Ophthalmology
Pittsburgh, Pennsylvania
October 1989 - August 1990
Maintained molecular biology lab studying the role of collagen and vimentin in corneal transplantation and wound healing of the eye in rabbit models

Performed SDS-PAGE electrophoresis, western blotting, HPLC, protein purifacation and column chromatography, animal surgery, tissue culture techniques and immunohistochemistry

Research Assistant II
University of Pittsburgh School of Medicine
Department of Pathology
Pittsburgh, Pennsylvania
May 1986 - October 1989
Worked in multiple laboratories performing experimental research in transplantation immunology of the liver and intestine with a focus on GVHD and tolerance induction through bone marrow chimera and immunosuppression (FK-506, cyclosporin).

Responsibilities included T-cell depletion, cytokine ELISA and lymphocyte proliferation assays, Mixed lymphocyte reaction, T-cell cytotoxicity, recombinant DNA techniques, liver perfusions, small animal surgery, FACS analysis of T-cell subsets.

Additional responsibilities included tissue culture of human and rat lymphocyte tumor cell lines, Lymphokine-activated killer (LAK) cell production and isolation, animal husbandry and skin grafting.

OTHER WORK EXPERIENCE

Recreation Supervisor
Carrboro Parks and Recreation
Carrboro, North Carolina
March 1992 - present
Supervisor, instructor and umpire for youth/adult baseball and softball, age groups 5 - 17
Design and instruction of coaching, umpire and baseball skills clinics for staff and players

Sales Associate
Old Navy Clothing
New Hope Commons
Durham, North Carolina
November 1995 - April 1999
HONORS AND AWARDS
PUBLICATIONS

Hoffmann, S.C., Bleiweis, M.S., Jones, Paik, H.C., Ciriaco, P., and D.R. Egan, T.M. Maintenance of cAMP in non-heart beating donor rat lungs reduces ischemia-reperfusion injury. Chest (submitted).

Hoffmann, S.C., Sadoff, J.D., and Egan, T.M. Effect of nitroglycerin (NTG) and cGMP on pulmonary endothelial integrity in non-heart beating rat lungs. Journal of Applied Physiology (submitted).

Thomas, Y.M., Hoffmann S.C., Gibson, D.L., Kiser, A.C., Paik, H.C., Funkhouser, W.K., Bleiweis, M.S., Ciriaco, P., and Egan, T.M. ICAM-1 adhesion molecule upregulation following lung transplantation from non-heart beating donors. American Journal of Respiratory Cell and Molecular Biology (in preparation).

Kiser, A.C. Hoffmann, S.C., Ciriaco, P., and Egan, T.M. Non-heart beating cadaver lung trasnplantation: Gas exchange and following low potassium dextran-glucose (LPDG) flushing in rats. Annals of Thoracic Surgery (Submitted).

Paik, H.C., Hoffmann, S.C., Kiser, A.C., and Egan, T.M. Pulmonary Preservation Solutions: Effects on endothelial function and pulmonary energetics. Transplantation (Submitted).

Bleiweis, M.S., Jones, D.R., Hoffmann, S.C., Egan, T.M. Reduced ischemia-reperfusion injury (IRI) with rolipram in rat cadaver lung donors: Effect of cAMP? Annals of Thoracic Surgery. 67(1) 194-200, January 1999.

Jones, D.R., Becker, R.M., Hoffmann, S.C., Lemasters, J.J., Egan, T.M. When Does the Lung Die?-Changes in Kfc, Cell Viability, and Adenine nucleotide levels in the circulation-arrested rat lung. J. Appl. Phys. 83(1) 247-252, July 1997.

Jones, D.R., Hoffmann, S.C., Sellars, M., Egan, T.M. Reduced Ischemia-Reperfusion Injury with Isoproterenol in Non-heart Beating Donor Lungs. J. Surg. Res. 69(2) 385-392, May 1997.

Hoffmann, S.C. Altered Expression of the p53 Tumor Suppressor Gene Enhances the Tumorigenic Potential of Neoplastically Transformed WB-F344 Rat Liver Stem-Like Epithelial Cells. Masters Thesis. University of North Carolina at Chapel Hill Collections, May 1995.

Markus P., Cai, C., Hoffman, A.L., Kunz, H.W., Hoffmann, S., Fung, J.J. Effect of thymus transplantation on allograft rejection in thymectomized bone marrow chimeras. Transplantation Proceedings 22(6) 2536-2538, December 1990.

Hoffman, A.L., Cai, X., Cramer, D.V., Makowka, L., Hoffmann, S.C., Starzl, T.E. Induction of stable chimerism and elimination of graft-versus-host disease by depletion of T-lymphocytes from bone marrow using immunomagnetic beads. Surgery 106(2) 354-363, August 1989.

ABSTRACTS

Kiser, A.C., Ciriaco, P., Hoffmann, S.C., and Egan, T.M. Lung retrieval from non-heart beating cadavers: assessment of gas exchange in a rat lung transplant model. Southern Thoracic Surgical Association, San Juan, Puerto Rico, November 4-6, 1999.

Paik, H., Hoffmann, S.C., Kiser, A.C., and Egan, T.M. Pulmonary preservation solutions: Effects on endothelial function and pulmonary energetics. American College of Chest Physicians, Toronto, Canada, November 8-12, 1998.

Bleiweis, M.S., Jones, D.R., Hoffmann, S.C., Ciriaco, P., and Egan, T.M. Maitenance of cAMP improves lung endothelial integrity in cadaver rat lungs. American College of Chest Physicians, Toronto, Canada, November 8-12, 1998.

Thomas, Y.A., Paik, H., Hoffmann, S.C., Ciriaco, P., and Egan, T.M. Are adhesion molecules upregulated after lung transplant from non-heart beating donors?. American College of Chest Physicians, Toronto, Canada, November 8-12, 1998.

Lucas, C., Mill, M., Lucas, W., Kiser, A., Ha, B., Henry, G.W., Yim, P., Hoffmann, S.C., Ketner, M., Masters, J., Wilcox, B., Yoganthan, A., Ensley, A., Lynch, P., Chatzinavroudis, G., Sharma, S. Hemodynamic perspectives on Fontan circulations. Third World Congress of Biomechanics. Sapporo, Japan, August 2-8, 1998.

Bleiweis, M.S., Jones, D.R., Hoffmann, S.C., Becker, R.M., and Egan, T.M. Reduced ischemia-reperfusion injury (IRI) with rolipram in rat cadaver lung donors: Effect of cAMP. Society of Thoracic Surgeons, January 26-28, 1998.

Jones, D.R., Hoffmann, S.C., Egan, T.M. Reduced ischemia-reperfusion injury (IRI) with isoproterenol in non-heart beating donor lungs. Association for Academic Surgery Annual Meeting, Chicago, Ill., November 13-16, 1996.

Hoffmann, S.C., Smith, G.J. Site-directed recombination mutagenesis (SDRM) as targeting gene therapy on the p53 tumor suppressor gene in WB-F344 rat stem cells. NIEHS: Environmental Carcinogenesis and Cancer Symposium. May 1993.

Hoffmann, S.C., Coleman, W.B., Smith, G.J. Alterations in the p53 tumor Suppressor gene in C3H10T1/2 cells in vitro. Program in Moelcular Biotechnology and Protein Engineering Symposium. May 1992.

ACTIVITIES AND INTERESTS

SEMI-PRO BASEBALL

GRADUATE AND PROFESSIONAL STUDENT FEDERATION (GPSF)