Oliver Smithies

Research and Professional Experience

1953-1960               Connaught Medical, Research Laboratory, University of Toronto, Canada
1960-1988               Assistant, Associate and Leon J. Cole and Hilldale Professor of Genetics and                                               Medical Genetics, University of Wisconsin-Madison
1988 to Present      Excellence Professor of Pathology and Laboratory Medicine,
                                University of North Carolina at Chapel Hill

Most Significant Publications

(numbering refers to complete publications list)

75.      Blattner, F. R., B. G. Williams, A. E. Blechl, K. Denniston-Thompson, H. E. Faber, L. A. Furlong, D. J. Grunwald, D. O. Kiefer, D. D. Moore, J. W. Schumm, E. L. Sheldon and O. Smithies.  Charon Phages for Cloning DNA:  Characterization and Safety Tests.  Science 196:161-169 (1977).


81.      Smithies, O., A. E. Blechl, K. Denniston-Thompson, N. Newell, J. E. Richards, J. L. Slightom, P. W. Tucker and F. R. Blattner.  Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA:  Characterization and Partial Sequencing.  Science 202:1284-1289 (1978).


108.    Smithies, O., R. G. Gregg, S. S. Boggs, M. A. Koralewski and R. S. Kucherlapati.  Insertion of DNA Sequences into the Human Chromosomal ß Globin Locus via Homologous Recombination.  Nature 317:230-234 (1985).


115.    Maeda, N. and O. Smithies.  The Evolution of Multigene Families:  Human Haptoglobin Genes.  Ann. Rev. Genet. 20:81-108 (1986).


121.    Doetschman, T., R. G. Gregg, N. Maeda, M. L. Hooper, D. W. Melton, S. Thompson and O. Smithies.  Targetted Correction of a Mutant HPRT Gene in Mouse Embryonic Stem Cells.  Nature 330:576-578 (1987).


129.    Koller, B. H., L. J. Hagemann, T. Doetschman, J. R. Hagaman, S. Huang, P. J. Williams, N. L. First, N. Maeda, and O. Smithies, Germ-line Transmission of a Planned Alteration Made in a Hypoxanthine Phosphoribosyltransferase Gene by Homologous Recombination in Embryonic Stem Cell. Proc. Natl. Acad. Sci. 86:8927-8931 (1989).


134.    Koller, B. H., P. Marrack, J. W. Kappler, and O. Smithies, Normal Development of Mice Lacking ß2M and Deficient in MHC Class I Proteins and CD8+ T Cells.  Science 248:1227-1230 (1990).


142.    Koller, B.H., H.-S. Kim, A. M. Latour, K. K. Brigman, R. C. Boucher, Jr., P. Scambler, B. Wainwright, O. Smithies, Toward an Animal Model of Cystic Fibrosis: Targeted Interruption of Exon 10 of the Cystic Fibrosis Transmembrane Regulator Gene in Embryonic Stem Cells. Proc. Natl. Acad. Sci. USA 88:10730-10734 (1991).


146.    Snouwaert, J. N., K. K. Brigman, A. M. Latour, N. N. Malouf, R. C. Boucher, Jr., O. Smithies, and B. H. Koller, An Animal Model for Cystic Fibrosis Made by Gene Targeting. Science 257:1083-1088 (1992).


154.    Lubahn, D. B., J. S. Moyer, T. S. Golding, J. F. Couse, K. S. Korach, and O. Smithies, Alteration of Reproductive Function but not Prenatal Sexual Development after Insertional Disruption of the Mouse Estrogen Receptor Gene. Proc. Natl. Acad. Sci. USA 90:11162-11166 (1993).


155.    Smithies, O. and H.-S. Kim, Targeted Gene Duplication and Disruption for Analyzing Quantitative Genetic Traits in Mice.  Proc. Natl. Acad. Sci. USA 91:3612-3615 (1994).

162.    Kim, H.-S., J.H. Krege, K.D. Kluckman, J.H. Hagaman, J.B. Hodgin, C.F. Best, J.C. Jennette, T.M. Coffman, N. Maeda, and O. Smithies, Genetic Control of Blood Pressure and the Angiotensinogen Locus.  Proc. Natl. Acad. Sci. 92:2735-2739 (1995).


165.    Smithies, O., Maeda, N., Gene targeting approaches to complex genetic diseases: Atherosclerosis and essential hypertension. Proc. Natl. Acad. Sci. 92:5266-5272 (1995).


182.    Krege, J.H., Kim, H.-S., Moyer, J.S., Jennette, J.C., Peng, L., Hiller, S.K., and Smithies, O. Angiotensin converting enzyme gene mutations, blood pressures and cardiovascular homeostasis, Hypertension 29:150-157 (1997).


201.      Krege, J.H., Hodgin, J.B., Couse, J.F., Enmark, E., Warner, M., Mahler, J.F., Sar, M., Korach, K.S., Gustafsson, J.-A., and Smithies, O. Generation and Reproductive Phenotypes of Mice Lacking Estrogen Receptor-β, Proc. Natl. Acad. Sci. USA 95:15677-15682 (1998).


211.    Kim, H.-S., Maeda, N., Oh, G.T., Fernandez, L.G., Gomez, R.A., and Smithies, O. Homeostasis in Mice with Genetically Decreased Angiotensinogen Is Primarily by an Increased Number of Renin-producing Cells, J. Biol. Chem. 274:14210-14217 (1999).


215.    Takahashi, N., and Smithies, O. Gene Targeting Approaches to Analyzing Hypertension, J. Am. Soc. Nephrol. 10:1598-1605 (1999).


238.         Smithies O, Forty years with homologous recombination. Nature Medicine 7(10):1083-6 (2001).

243.   Kim, H.-S., Lee, G., John, S.W.M., Maeda, N., and Smithies, O. Molecular phenotyping for analyzing subtle genetic effects in mice: Application to an angiotensinogen gene titration. Proc. Natl. Acad. Sci. USA 99:4602-4607 (2002).


244.      Takahashi N, Brooks HL, Wade JB, Liu W, Kondo Y, Ito S, Knepper MA, and Smithies O. Posttranscriptional Compensation for Heterozygous Disruption of the Kidney-Specific NaK2Cl Cotransporter Gene. J Am Soc. Nephrol. 13:604-610 (2002).


245.   Ellmers L.J., Knowles J.W., Kim H.-S., Smithies O., Maeda N., and Cameron V.A. Ventricular expression of natriuretic peptides in Npr1-/- mice with cardiac hypertrophy and fibrosis. Am. J. Physiol. Heart Circ. Physiol. 283:H707-H714 (2002).


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