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Protocols Developed in the Rogers Lab

S2 Cell Protocols
How to culture S2 cells for RNAi and immunofluorescence studies.

D17 Cell Protocols
How to culture D17 cells for studies of cell migration.

S2 Purification of GST-tagged recombinant proteins

Purification of 6xHis-tagged recombinant proteins

Electroporation of S2 cells
How to transfect S2 cells using an Amaxa Nucleofector with home-made reagents.

Transfection of HEK cells using PEI
How to perform high-efficiency HEK cell transfection with home-made PEI reagent.

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Lab News

Fall 2011
* Alyssa passed her graduate Preliminary Exam

Spring 2011
* Kyle was awarded a prestigious fellowship from the American Heart Association
* Congratulations to Dr. Josh Currie on his thesis defense!!
* Josh's paper was accepted for publication in Nature Protocols
* Check out our new paper about the role of Katanin in cell migration in Nature Cell Biology

Contact

Rogers Lab
Department of Biology
The University of North Carolina at Chapel Hill
422 Fordham Hall, Campus Box 3280
Chapel Hill, NC 27599-3280

Tel: 919.843.7788
Fax: 919.962.1625

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