The antibodies are mouse monoclonal and are provided as 50% ascites fluid in glycerol with azide as preservative. Available antibodies are listed below along with pertinent information such as IgG class, approximate binding site, and suggested working dilutions for common applications.

More antibodies will be added to this list as they become available. Please check back often for updates.

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If you'd like to see representative images of blots, immunostaining, or a sequence alignment of the epitopes with some major species, click the buttons below.

For those interested in phosphorylation sensitive antibodies as discribed in Hegedus et al. (2009) (ref #12). The antibodies are identified by their respective serine sites in the paper and correspond to antibodies 217,432,450, and 570 below. Westerns can be found in the Western blot section.

 

Antibody

Comments

References

TJA9

Class: IgG1
Suggested Dilutions:
Western Blot : 1:500+
Cytochemistry : Not tested
Domain Recognized: EL1
(aa 103-117)


154

Class: IgG2a
Suggested Dilutions:
Western Blot : 1:500
Cytochemistry : TBD
Domain Recognized: RI region of NBD1
(aa 405-436)

660

Class: IgG2b
Suggested Dilutions:
Western Blot : 1:500 to 1:2000
Cytochemistry : TBD
Domain Recognized: NBD1
(aa 576-585)

1


8H16

Class: TBD
Suggested Dilutions:
Western Blot : 1:250
Cytochemistry : TBD
Domain Recognized: R domain
(aa 648-657)

1I22

Class: IgG2b
Suggested Dilutions:
Western Blot : 1:500+
Cytochemistry : 1:250+
Domain Recognized: R-Domain
(aa 668-675)

450

Class: IgG1
Suggested Dilutions:
Western Blot : 1:1000 to 1:5000
Cytochemistry : 1:250
Domain Recognized: R Domain
( aa 696-705)
epitope quenched by pKA phosphorylation

10,12

570

Class: IgG1
Suggested Dilutions:
Western Blot : 1:1000 to 1:5000
Cytochemistry : 1:250
Domain Recognized: R Domain
(aa 731-742)
epitope quenched by pKA phosphorylation

1,3,4,7,8,11,12

432

Class: IgG1
Suggested Dilutions:
Western Blot : 1:1000 to 1:5000
Cytochemistry : 1:250
Domain Recognized: R Domain
(aa 762-770)
epitope quenched by pKA phosphorylation

12

217

Class: IgG1
Suggested Dilutions:
Western Blot : 1:1000 to 1:5000
Cytochemistry : TBD
Domain Recognized: R Domain
(aa 807-819)
epitope quenched by pKA phosphorylation

1,7,8,12

596

Class: IgG2b
Suggested Dilutions:
Western Blot : 1:1000 to 1:5000
Cytochemistry : 1:250
Domain Recognized: NBD2
(aa 1204-1211)

1-9,11,12

769

Class: IgG1
Suggested Dilutions:
Western Blot : 1:1000 to 1:5000
Cytochemistry : 1:250
Domain Recognized: NBD2
(aa 1204-1211)

7,8

528

Class: IgG1k
Suggested Dilutions:
Western Blot : 1:1000+
Cytochemistry : 1:250+
Domain Recognized: NBD2
(aa 1204-1211)